Mouse Recombinant CA14 (from HEK293 cells), His Tag

Supplier: Avantor

Supplier: Avantor

About this item

A DNA sequence encoding the extracellular domain of mouse CA14 (NP_035927.1) (Met 1-Met 290) was expressed with a C-terminal polyhistidine tag.

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Specifications

Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Mouse
Tag sequence:
His Tag
Storage Conditions:
Store it under sterile conditions at –20 °C to –80 °C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Content:
< 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity:
Measured by its esterase activity. The specific activity is >400 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 0.8 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
Gene ID:
NP_035927.1
Reconstitution Instructions:
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Protein/Peptide Name:
CA14
Purity:
> 95 % as determined by SDS-PAGE
Molecular Weight:
The recombinant mouse CA14 consists of 286 amino acids and has a calculated molecular mass of 32.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 45-48 kDa protein in SDS-PAGE under reducing conditions.
Sequence:
Met1-Met290
Formulation:
Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.5
Shipping Temperature:
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.