Human Recombinant FCGR2A (from HEK293 cells), His Tag

Supplier: Sino Biological

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Supplier: Sino Biological

About this item

A DNA sequence encoding the extracellular domain (Met 1-Ile 218) of human CD32a (AAA35827.1) precursor was expressed, with a C-terminal polyhistidine tag. It is identical to FCGR2A131H/R in the reference.

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Specifications

Conjugation:
Unconjugated
Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Human
Storage Conditions:
–20…–80 °C
Endotoxin Content:
<1.0 EU per μg of the protein as determined by the LAL method
Biological Activity:
1. Measured by its ability to bind biotinylated human IgG2 (Cat: 13504-HNAH) in functional ELISA.
2. Loaded Anti-CD32A Antibody, Mouse Monoclonal(Cat. 10374-MM02) on AMC Biosensor, can bind CD32A Protein, Human, Recombinant (167 Arg, His Tag)(Cat. 10374-H08H) with an affinity constant of 6.8 nM as determined by Octet RED System.
Gene ID:
P12318-1
Reconstitution Instructions:
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Carrier-Free:
Y
Animal-Free:
Y
Protein Synonyms:
CD32|FcGR|CD32A|IGFR2|Fc gamma RIIA|FCG2|FCGR2A1|CDw32|FCGR2
Protein/Peptide Name:
FCGR2A
Purity:
>95% as determined by SDS-PAGE. >90% as determined by SEC-HPLC.
Molecular Weight:
The recombinant human FCGR2A consists of 196 amino acids after removal of the signal peptide and predicts a molecular mass of 22 kDa. It migrates as an approximately 30 kDa protein due to glycosylation by SDS-PAGE under reducing conditions.
Sequence:
Met1-Ile218,167R
Shipping Temperature:
Ambient
Protein Function:
Immunotherapy
Tag sequence:
His Tag
Formulation:
Lyophilized from sterile PBS, pH 7.5