DMEM F-12 medium contains 2.5 mM L-glutamine, 15 mM HEPES, 0.5 mM sodium pyruvate, and 1200 mg/l sodium bicarbonate.

• Store medium at 2 °C to 8 °C in the dark when not in use
• pH: 7.0 to 7.4
• Osmolality: 285 to 365 mOsm/kg
• Endotoxin: ≤0.5 EU/ml
• Sterility tests are performed using USP methods

Dulbecco's Modified Eagle's Medium (DMEM), ATCC® 30-2006™