2XYT-Broth is a nutritional rich medium providing nitrogen and growth factors optimized for the growth of recombinant E. coli strains infected with the M13 bacteriophage or other filamentous single stranded DNA bacteriophages thus allowing the bacteriophage to reproduce in large quantities without exhausting the host cells.

• 31 g (31 capsules or 6 large capsules) per liter
• Contents/l: 16 g, tryptone, 10 g yeast extract, 5 g NaCl bacterial growth medium
• Medium optimized for the growth and maintenance of M13 or other filamentous phage

Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in 2xYT Broth formulations. Chosen reagents are combined in the accurate proportions according to the formula listed above and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid. The combinations were mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.

Application includes: Bacteria cell culture, gene expression, transformation, microbiological analysis.