Simple, complete differentiation assay.

• CFUs are formed in semi-solid methylcellulose

Target populations: Granulocyte and macrophage, Granulocyte, macrophage.

It is a miniaturized, in vitro colony-forming cell (CFC) or unit (CFU) differentiation assay that detects the differentiation ability and/or potential of lympho-hematopoietic stem, progenitor or precursor cells to form colonies in semi-solid methylcellulose.

StemfateTM-4 assay utilizes specially designed 35mm petri dishes containing 4 mini-wells (0.1 ml each) which allow colonies to be easily viewed, differentiated and counted up to the edge of the well. The StemFateTM-4 assay kit includes a superior Master Mix to support optimal growth and enumeration of your lympho-hematopoietic cell population of interest, including erythroid progenitors (CFU-E and BFU-E), granulocyte/macrophage progenitors (CFC-GM, CFU-M, CFU-G), multi-potential granulocyte, erythroid, macrophage and megakaryocyte progenitors (CFU-GEMM), and more in just 10 to 14 days.