Quantitative determination of maltose in various biological samples such as serum, urine, food and beverages, and more.

• Fast and sensitive, convenient
• Use of 10 µl sample
• Linear detection range 2 to 500 µM maltose in for colorimetric assays and 1 to 50 µM for flourimetric assays
• Room temperature assay
• No 37 °C heater is needed
• High-throughput, "Add-mix-read" type assay
• Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
• Method: OD570 nm, or FL530/585 nm

The procedure involves adding a single working reagent, and reading the absorbance after 60 minutes. Maltose (C₁₂H₂₂O₁₁) is a disaccharide, composed of two glucose units linked by an α bond. It is produced from the hydrolysis of glycogen or starch, serving as a source of energy for plants and animals.

Maltose can be found in foods such as grains, and other processed products. Maltose assay provides a simple, one step assay for measuring maltose. In this assay, maltose is converted to two glucoses, which are then oxidized to form a colored product. The color intensity of the product at 570 nm or fluorescence at λex/em = 530/585 nm is directly proportional to maltose concentration in the sample. Samples: Serum, urine, food and beverages, and more.