Acid Phosphatase activity determination in biological samples (example: plasma, serum, cell lysate, tissue samples.)

• Fast and sensitive
• Linear detection range (20 μl sample): 0.05 to 60 U/l for a 30 minute reaction
• High-throughput,homogeneous "mix-incubate-measure" type assay
• Can be readily automated on HTS liquid handling systems for processing thousands of samples per day
• Method: OD405 nm
• Samples: Biological (example serum) samples

Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.

Non-radioactive, colorimetric ACP assay is based on the cleavage of p-nitrophenol from the synthetic substrate. p-Nitrophenol becomes intensely colored after addition of the stop reagent. The increase in absorbance at 405 nm after addition of the stop reagent is directly proportional to the enzyme activity.