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AnaTag™ B-PE Labeling Kit, Anaspec Inc.
AnaTag™ B-PE Labeling Kit, Anaspec Inc.
Catalog # 103010-182
Supplier:  Anaspec Inc
CAS Number:  
AnaTag™ B-PE Labeling Kit, Anaspec Inc.
Catalog # 103010-182
Supplier:  Anaspec Inc
Supplier Number:  AS-72112
CAS Number:  

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Specifications

  • Description:
    AnaTag™ B-PE Labeling Kit
  • Size:
    1 kit
  • Storage Conditions:
    4°C
  • Shipping Conditions:
    20
  • Cat. No.:
    103010-182
  • Abs/Em:
    545,563/578 nm
  • No. of reactions:
    1 labeling reaction (1x1 mg IgG)
  • Supplier no.:
    AS-72112

Specifications

About this item

The AnaTag™ B-PE Labeling Kit is optimized for use in the conjugation of B-Phycoerythrin (B-PE) to antibodies. B-PE, a fluorescent protein from phycobiliprotein family, has a primary absorption peak at 545 nm with a secondary peak at 563 nm and maximum emission at 578 nm.

B-PE and the closely related R-PE are the most intensely fluorescent phycobiliproteins having orange fluorescence. They are significantly brighter and more photostable than conventional organic fluorophores. B-PE labeled primary and secondary antibodies are used in applications such as flow cytometry and multi-color immunofluorescent staining. The AnaTagTM B-PE Labeling Kit contains SH-reactive B-PE. SMCC modified B-PE reacts with the thiol groups of target antibody without the need for additional activation, thus simplifying the conjugation protocol. The amount of B-PE supplied in this kit is sufficient for labeling up to 1 mg of antibody. The kit provides all reagents, purification columns and a detailed step-by-step protocol. Native and SMCC-activated B-PE are also available as separate products.