The T2/HT2 determination in food and feed focuses on extract using immunoaffinity column (IAC).

• Sensitivity: 0.2 to 400 ng
• Dynamic range: 0.2 to 400 ng
• Sample size: 10 columns

This instruction of T2/HT2 determination in food and feed focuses on the enrichment step of extract using immunoaffinity column (IAC) and quantification with HPLC. Accepted laboratory extraction methods could be maintained. Full performance of the IAC column is given if pronounced criteria regarding organic solvent tolerance, elution process of analyte and working range of column is followed. Many pretreatment methods for T2/HT2 in food and feed show low sensitivity because of interferring substances if problematic matrices are applied. This method of content determination of T2/HT2 combines the high selectivity of an immunoaffinity column (IAC) with its potential to concentrate elute and additional step of purification of labelled T2/HT2 by HPLC column. As said before, this instruction focuses on the handling with the IAC column. For the commodity extraction step a literature method is given. Please see below. The given apparatus (e.g. HPLC system) might serve as example among other possibilities