The fumonisins determination in feed focuses on extract using immunoaffinity column (IAC).

• Sensitivity: 0.2 to 400 ng FUM
• Dynamic range: 0.2 to 400 ng FUM
• Sample size: 10 columns

This instruction of fumonisins (FB1 / FB2) determination in food and feed focuses on the enrichment step of extract using immunoaffinity column (IAC) and quantification with HPLC. Accepted laboratory extraction methods could be maintained. Full performance of the IAC column is given if pronounced criteria regarding organic solvent tolerance, elution process of analyte and working range of column are followed. Many pretreatment methods of fumonisins determination in food and feed, most of them which are based on solid phase extraction (SPE) with adsorbents or strong anion resins (SAX), show low sensitivity because of interfering substances if problematic matrices are applied.