IAC use in clean-up of single mycotoxins of complex feed extracts is well known in lab work.

• Sensitivity: 0.02 ng AFL, 0.02 to 2 ng OTA, 0.2 to 20 ng ZON,1 to 350 ng DON,0.2 to 400 ng FUM, 0.2 to 400 ng T2/HT2
• Dynamic Range: 0.02 to 3 ng AFL, 0.02 to 2 ng OTA, 0.2 to 20 ng ZON, 1 to 350 ng DON ,0.2 to 400 ng FUM, 0.2 to 400 ng T2/HT2
• Sample Size: 10 columns

The use of immunoaffinity columns (IAC) in the clean-up of single mycotoxins of complex food and feed extracts is well established in laboratory work. This especially applies for the determination of mycotoxins which limits are regulated by authorities; that are aflatoxins, ochratoxins, zearalenon, deoxynivalenol, fumonisins and T2-toxin. B-TeZ IAC Combi AOZDFT 3 ml, the new Multianalyte-IAC, offers the user a tool to combine high purification potential of IAC, that means less matrix effects in apparatus even for difficult matrices, with multianalyte function of LC-MS/MS.

The most important mycotoxins, which are regulated by authorities, can be determined in parallel in one single run, that comprises extraction - enrichment - LC-MS/MS-measurement. Maximum levels set by European Commission /20061 are met for all commodity samples. The same IAC protocol can be used for all commodities, except for baby food where a bigger aliquot of sample extract should be used because of very low maximum levels of aflatoxin and ochratoxin in that commodity.