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Specifications
- Description:functional IGFBP-3 LIA
- Size:1 kit
- Cat. No.:102994-274
- Supplier no.:E04A
Specifications
About this item
The Eagle Biosciences IGFBP-3 LIA utilizes specific and high affinity antibodies for IGFBP-3.
- Sensitivity: 0.4 ng/mL
- Dynamic Range: 0.4 - 30 ng/mL
- Incubation Time: 3.5 hours
All currently existing IGFBP-3 immunoassays use the binding of specific anti-IGFBP-3 antibodies for signal generation and thus IGFBP-3 quantification. The failure of differentiation between complete IGFBP-3 molecules and their respective fragments (derived physiologically due to the different proteases activities) is unavoidable in this system. Because one molecule IGFBP-3 can be cleaved in several fragments often false high quantitative values are measured. Based on this methodology it is not possible to differentiate between high IGFBP-3 levels in fact, or, a high degree of fragmentation. The incidental attempts to use monoclonal antibodies with a binding region represented only by the intact IGFBP-3 molecule are indirect, imprecise and insufficient. The activities of all effective proteases, which have different sites of action and therefore generate different kind of fragments, are disregarded.