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- Size:20 μg
- Cat. No.:PAL1061
- Storage temperature:–30...–10 °C
- Supplier no.:L1061
Designed for use with Insect Cell Extracts and contain untranslated region (UTR) sequences at the 5´ and 3´ ends of the gene coding region to enhance translation efficiency.
- Protein Expression Vectors Used With Insect Cell Extracts for In Vitro Translation
- Efficient transfer of Flexi(R) vector inserts for direct use of recombinant clones, minimizing time wasted screening background colonies
- Adaptable to high-throughput formats for large screening projects
- Transfer your cloned insert into other Flexi(R) Vectors for additional applications (e.g., bacterial or mammalian protein expression) as needed
The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi Vectors without the need to resequence. The vectors are designed with special sequences for maximal cell-free protein expression in a specific system. The pF25A/K ICE Vectors were designed for use with Insect Cell Extracts and contain untranslated region (UTR) sequences at the 5´ and 3´ ends of the gene coding region to enhance translation efficiency. Note: Flexi Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.