To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
- Size:20 μg
- Environmentally Preferable:
- Cat. No.:PAG2751
- Storage temperature:–30...–10 °C
- Supplier no.:G2751
These Flexi System-based vectors are used for inducible bacterial expression of N-terminal HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.
- N-Terminal HaloTag(R) Fusion Bacterial Expression Vectors
- Expresses proteins in E. coli and T7 cell-free systems
- Barnase insert decreases the number of background colonies for efficient transfer of genetic constructs
- Choose from Ampicillin and Kanamycin resistance
The following vectors are used for inducible expression of HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Expression levels depend highly on the nature of the protein, but in general the N-terminal HaloTag fusion protein (e.g., pFN18A/K, Cat.# G2751, G2681) can increase expression level, enhance refolding and boost solubility of the expressed protein. HaloTag vectors are Flexi System vectors. The Flexi Vector System is a simple, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi Vectors without the need to resequence. Direct transfers can only occur between two N-terminal tagged vectors or from an N-terminal to a C-terminal vector. pFN18A/K HaloTag T7 Flexi Vectors (Cat.# G2751, G2681) are designed for protein expression with an N-terminal HaloTag in E. coli and T7 cell-free expression systems. Note: Flexi Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert.