pGL4.73[hRluc/SV40], pGL4.74[hRluc/TK] and pGL4.75[hRluc/CMV] Vectors are used as the control reporter for dual-luciferase assays. Select the appropriate promoter or test all three in your experimental system.

• Vectors Co-transfected in Dual-Reporter Assays as the Control Vector
• Co-transfect into cells where firefly luciferase is the experimental reporter gene
• CMV, SV40 and TK promoter options offer different expression levels suitable for most dual-reporter assays

The Promoter-driven Renilla control vectors are commonly co-transfected with experimental firefly luciferase vectors for use in the Dual-Luciferase or Dual-Glo Reporter Assay Systems. The control Renilla vectors should give an almost invariant level of activity, while the experimental firefly vector varies with treatment.