pBlueScript II Vectors, Agilent Technologies

The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.

High copy number ColE1-based phagemid
Large and versatile polylinker in two orientations, f1 origin available in either (+) or (–) orientation
T3 and T7 promoters for in vitro transcription of RNA

The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites.

: For research use only. Not for use in diagnostic procedures.

High copy number ColE1-based phagemid
Large and versatile polylinker in two orientations, f1 origin available in either (+) or (–) orientation
T3 and T7 promoters for in vitro transcription of RNA

The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites.

: For research use only. Not for use in diagnostic procedures.

Product Family Options

Description
Size
Details
95040-830

pBluescript II KS(+) Phagemid Kit
1 Kit
f1 origin in (+) orientation, Kpn-Sac polylinker orientation. Contains: 20 μg pBluescript II KS(+) phagemid vector, host strain: XL1-Blue MRF'

Each
Each - $560.37
$560.37
Each
$560.37
: 212207
99900-360
pBluescript II KS(–) Phagemid Kit
1 Kit
f1 origin in (−) orientation, Kpn-Sac polylinker orientation. Contains: 20 ug pBluescript II KS(−) phagemid vector, host strain: XL1-Blue MRF'

-
: -
95040-832

pBluescript II SK(+) Phagemid Kit
1 Kit
f1 origin in (+) orientation, Sac-Kpn polylinker orientation. Contains: 20 μg pBluescript II SK(+) phagemid vector, host strain: XL1-Blue MRF'

Each
Each - $560.37
$560.37
Each
$560.37
: 212205
99900-342
pBluescript II SK(–) Phagemid Kit
1 Kit
f1 origin in (−) orientation, Sac-Kpn, polylinker orientation. Contains: 20 μg pBluescript II SK(−) phagemid vector, host strain: XL1-Blue MRF'

-
: -
99900-444
pBluescript II RI Predigested Vector
55 Reactions
Predigested with EcoR I

-
: -
95040-826

pBluescript II XR Predigested Vector
55 Reactions
XR vector is predigested with Xho I and EcoR I

-
: -
95040-870
R408 Interference Resistant Helper Phage
1 ml
Stable interference-resistant helper phage (4 kB, single stranded). For preferential secretion of single-stranded vectors containing intact segments of an M13 intergenic region over the helper phage DNA

-
: -
ST200251
VCSM13 Interference Resistant Helper Phage
1 ml
Interference-resistant helper phage (6 kB, single stranded). Derived from M13 K07 mutant. Carries kanamycin-resistance gene in IG region. High rescue efficiencies with kanamycin selection

Each
Each - $295.69
$295.69
Each
$295.69
: 200251

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