Transfection Carrier DNA, Promega
PAE4881
:
- Description:Transfection Carrier DNA, 5 x 20microg
- Size:5 x 20 μg
- Environmentally Preferable:
- Storage Temperature:-30°C to -10°C
- Cat. No.:PAE4881
- Supplier no.:E4881
A plasmid DNA used to reduce the amount of an expression vector or reporter vector in mammalian cell transfection without reducing the overall amount of DNA.
- Plasmid DNA Used to Dilute Expression or Reporter Vectors for Transfection
- Maintains the total amount of DNA used in transfection while reducing the amount of expression or reporter vector
- Lowered expression of the protein of interest can overcome potential overexpression artifacts
- Ratio of carrier DNA to reporter DNA may need to be optimized, depending on the application
- Supplied at a concentration of 1µg/microl
A plasmid DNA used to reduce the amount of an expression vector or reporter vector in mammalian cell transfection without reducing the overall amount of DNA. Dilution of reporter DNA into Transfection Carrier DNA results in lowered expression of the protein of interest, overcoming artifacts due to overexpression. This is especially relevant when strong, constitutive promoters (e.g., CMV) are used. Depending on the applicaiton, the ratio of reporter to Transfection Carrier DNA may require optimization.