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Specifications
- Description:Single-Use HB101 Competent Cells, >10(8)cfu/ug, 1ml
- Size:1ml
- Environmentally Preferable:
- Storage Conditions:LESS THAN -65°C
- Cat. No.:PAL2015
- Supplier no.:L2015
Specifications
About this item
HB101 cells are recA- to minimize recombination events and contain a restriction-minus background (hsdS20) to allow better representation when cloning methylated DNA. Suitable for vectors that do not require alpha-complementation for blue/white screening.
- Ready-to-Use Competent E. coli Cells for Recombinant Cloning and Subcloning
- Cells are ready to use—no preparation time necessary
- Transformation efficiencies guaranteed
- No worries about undesirable recombination events (recA- mutation)
HB101 cells are recA-, which minimizes recombination events, and contain a restriction-minus background (hsdS20) that allows better representation when cloning methylated DNA. HB101 Competent Cells are suitable for vectors that do not require alpha-complementation for blue/white screening. For researchers doing more than one transformation, competent cells are available in standard format (200ul aliquots). For added convenience, single-use competent cells (50ul aliquots) also are offered. HB101 Competent Cells are prepared according to a modified procedure of Hanahan. HB101 Genotype: F-, thi-1, hsdS20 (r(B)-, m(B)-), supE44, recA13, ara-14, leuB6, proA2, lacY1, galK2, rpsL20 (str(r)), xyl-5, mtl-1.