Created by digesting lambda DNA to completion with EcoRI and HindIII to generate 13 fragments of 125-21,226bp.

• Thirteen DNA Fragments of 125-21,226bp
• Load 1microl/lane
• Supplied at a concentration of 0.5µg/microl
• Supplied with 6X Blue/Orange Loading Dye

The Conventional DNA Digest Markers are created by digesting either lambda DNA, PhiX174 replicative form DNA, or plasmids to completion with one or more restriction enzymes. The enzymes are heat-inactivated, and the DNA fragments are either phenol-extracted, then ethanol-precipitated or just ethanol-precipitated. The precipitated fragments are resuspended in storage buffer. The markers are not intended for quantitative analysis. Each marker is supplied with a tube of 6X Blue/Orange Loading Dye. Lambda DNA/EcoRI + HindIII Markers: Thirteen ethanol-precipitated DNA fragments ranging in size from 125bp to 21,226bp. Recommended loading: 1microl/lane