dUTP (2'-Deoxyuridine, 5'-Triphosphate) can be used in place of dTTP in PCR and RT-PCR to prevent carryover from previous amplifications.

• Prevent Carryover from Previous PCR Amplifications
• Highly pure (≥99% triphosphate)
• Supplied at 100mM in water for ease-of-use
• Incorporate dUTP into PCR products and treat with uracil-N-glycosylase to inhibit subsequent amplifications

High-quality deoxynucleotide triphosphates (dNTPs) are critical for the success of many key procedures such as cDNA synthesis, sequencing and labeling. Promega dNTPs have greater than 99% triphosphate content and are provided at a concentration of 100mM in water at pH 7.5. dUTP (2'-Deoxyuridine, 5'-Triphosphate) can be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. The substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme uracil-N-glycosylase (UNG, also referred to as UDG) can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Each lot of dUTP is function-tested to ensure specific DNA amplification and the absence of nuclease activity.