To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
- Description:Bacterial Strain JM109(DE3)
- Size:500 μl
- Cat. No.:PAP9801
- Supplier no.:P9801
Used for high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, if the sequence contains a ribosomal binding site. No blue/white selection.
- For High-Level Gene Expression from the T7 Promoter
- Contains an IPTG-inducible gene for T7 RNA polymerase
- Requires a ribosomal binding site with cloned sequence
- Lacks blue/white cloning selection
Bacterial Strain JM109(DE3), derived from JM109, contains a chromosomal copy of the gene for T7 RNA polymerase. JM109(DE3) is used for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, provided that the cloned sequence contains a ribosomal binding site. Please note: JM109(DE3) cannot be used for blue/white selection. Genotype: endA1, recA1, gyrA96, thi, hsdR17 (r(k)-, m(k)+), relA1, supE44, lambda-, Delta(lac-proAB), [F', traD36, proAB, lacI(q)ZDeltaM15], lDE3.