To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
- Application:
- Description:Bacterial Strain ES1301 mutS
- Size:200 μl
- Environmentally Preferable:
- Cat. No.:PAQ6131
- Supplier no.:Q6131
Mismatch repair minus strain.
- Mismatch Repair Minus Strains of E. coli
- Prevents repair of the newly synthesized unmethylated DNA strand
- ES1301 is Tets for cycling between antibiotic resistances
- BMH 71-18 is Tetr and can only be used to select Ampr or CATr mutants
BMH 71-18 mutS and ES1301 mutS are mismatch repair minus strains of E. coli. Use of these strains prevents repair of the newly synthesized unmethylated strand, leading to high mutation efficiencies. Both ES1301 and BMH 71-18 mutS are recA+, therefore inserts containing highly repetitive sequences may be unstable. ES1301 is Tet(s), which allows for cycling between antibiotic resistances. BMH 71-18 is Tet(r); therefore, it can only be used to select Amp(r) or CAT(r) mutants. ES1301 mutS Genotype: lacZ53, mutS201::Tn5, thyA36, rha-5, metB1, deoC, IN(rrnD-rrnE). BMH 71-18 mutS Genotype: thi, supE, Delta(lac-proAB), [mutS::Tn10], [F', proAB, lacI(q)ZDeltaM15].