DMNPE-caged Luciferin

The cell permeable DMNPE-caged Luciferin is as a bioluminescent luciferase substrate used to measure intracellular functions since it readily crosses cell membranes. It is a D-luciferin ester analogue of luciferin.

• High purity
• Cell permeable: efficient luciferin delivery into intact cells even at neutral pH
• Ideal for in vivo applications
• Induces prolonged release of luciferin by the action of cells’ esterases
• Allows to follow changes in gene expression in live cells
• Suitable for in vitro experiments

Once the caged luciferin is inside the cells, active luciferin can be released by the action of endogenous intracellular esterases or by a flash of UV light. This implies:
1. A more efficient delivery of luciferin into intact living cells & improved sensitivity analysis of in vivo luciferase assays
2. A long-term measurement of luciferase activity since hydrolysis by esterases allows a continuous supply of active Luciferin
DMNPE-caged luciferin appears a good alternative to D-Luciferin classically used to measure luciferase activity in live cells. Indeed, the difficulty to deliver D-Luciferin into living cells renders in vivo quantification limited to a certain extent- for example, permeability issues at neurtral pH.

Shipped at room temperature.
Store at -20°C and protect from light.