To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
Specifications
- Application:
- Description:pSTBlue-1 Blunt® Giga cloning kit
- Size:20 rxn
- Environmentally Preferable:
- Cat. No.:EM71229-3
- No. of reactions:20
- Supplier no.:71229-3
Specifications
About this item
The pSTBlue-1 Perfectly Blunt® Giga Cloning Kits feature NovaBlue GigaSingles™ Competent Cells for increased transformation efficiency (>1×109 cfu/µg). NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue-white screening capability (with appropriate plasmids) and recA endA mutations, which facilitate high yields of excellent-quality plasmid DNA. Cells are provided in a 50 µL, single-use volume that eliminates the need to dispense, freeze/thaw or waste partially used vials.
- No restriction enzymes or special primers
- Compatible with any DNA polymerase
- Independent of 3ʹ-dA addition or sequence context of DNA ends
- Blue/white screening with seven different vectors, including pETBlue expression vectors
- Simple protocol takes less than one hour from PCR product to plating transformants
The Perfectly Blunt® Cloning Kits are designed for simplified cloning of DNA generated by PCR using any type of DNA polymerase. This approach enables the use of high-fidelity proofreading enzymes for amplification, thus decreasing the probability of generating mutations in the target sequence. In addition, under many conditions blunt cloning is more efficient than T-cloning, most likely due to the observation that the efficiency of single dA addition by Taq DNA polymerase varies significantly depending on the sequence context of the DNA ends, and even the number of PCR cycles performed.
These kits are also suitable for cloning restriction fragments, cDNA, or sheared DNA with the same protocols.
Storage: –70 °C