ZR Plasmid MiniPrep™ Purification Kits, Zymo Research

About this item

ZR Plasmid MiniPrep™ Classic is designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol together with a unique fast-spin column to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy determination of complete cell lysis and neutralization. Plasmid DNA purified using this kit is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.

Easy traditional method for isolating high-quality plasmid DNA from E. coli
Unique column design: zero buffer retention and low (30 µL) elution volume
Innovative colored P1, P2, and P3 buffers for rapid identification of complete bacterial cell lysis and neutralization steps

This kit yields up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. It is applicable for ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications.

Delivery: Kit includes Zymo-Spin™ IIN, DNA Elution Buffer (4 mL, 10 mL and 16 mL), Buffer (P1, P2, P3), Plasmid Wash Buffer (24 mL and 48 mL), Endo-Wash Buffer (30 mL), Endo-Wash Buffer (60 mL) and Collection Tubes (2 mL).

Restricted Products: To process your orders without delay, please provide the required business documentation to purchase this product.

To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:

• State issued document with your organization's Federal Tax ID Number
• State issued document with your organization's Resale Tax ID Number
• City or County issued Business License
• State Department of Health Services License
• Any other ID issued by the State that includes the business name & address

* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.