A simple, reliable, silica-resin-based method for isolation of plasmid DNA on a maxiprep scale. No organic extraction or ethanol precipitation required. Yields 300ug-1mg from 100-500ml culture.

• 300µg-1mg Plasmid DNA from 100-500ml Cultures
• 3-hour protocol
• Resin-based batch-binding and column-washing method
• No organic extractions

The Wizard Plus Maxipreps DNA Purification System provides a simple and rapid resin-based batch column method for purification of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. Use of this system requires only a centrifuge, a vacuum source and a vacuum manifold. This system typically yields 300ug-1mg of high-copy-number plasmid DNA (200-20,000bp) from a 100-500ml culture in less than three hours. The purified DNA is eluted in Nuclease-Free Water (Cat.# P1193) and can be used directly for DNA sequencing and restriction digestion without further manipulation. The DNA also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor such as Recombinant RNasin Ribonuclease Inhibitor (Cat.# N2511).