- Application:RT-qPCR probe
- Description:GoTaq Probe 2-Step RT-qPCR System
- Cat. No.:PAA6110
- No. of reactions:20 µl× 50 RT and 50 µl× 80 qPCR at 50 µl, 20 µl× 50 RT and 20 µl× 200 qPCR at 20 µl, 20 µl× 50 RT and 10 µl× 400 qPCR at 10 µl
- Volume:2 ml
- Supplier no.:A6110
GoTaq Probe RT-qPCR Systems enable detection and relative quantification of RNA expression levels.
- Robust System to Simplify RNA Expression Analysis
- Integrated components for reverse transcription and downstream qPCR, including RNasin(R) Ribonuclease Inhibitor
- Compatible with both fast and standard cycling methods using probe-based assays
- Sensitive detection for quantitation over a broad dynamic range
The GoTaq Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript Reverse Transcription System and GoTaq Probe qPCR Master Mix. The GoScript Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions. The GoTaq Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript Reverse Transcriptase and GoTaq Probe qPCR Master Mix in a single-step real-time amplification reaction.