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Specifications
- Description:ROS Detection Assay Kit
- Environmentally Preferable:
- Brand Name:OZBI
- No. of transfections/assays:300 assays
- Cat. No.:103549-582
- Buffer volume:5 ml
- Volume:35uL DFC-DA in DMF + 50uL TBHP
Specifications
About this item
The ROS Detection Assay kit (Green Fluorescence) is a cell-based assay designed for measuring Reactive Oxygen Species (ROS) activity within a cell using the cell-permeable fluorogenic probe 2’, 7’-Dichlorodihydrofluorescein diacetate (DCF-DA).
- Measure ROS production in live cells
- Ideal for fluorescence microscopy, microplate titration and cytometry
- Compatible with adherent cells as well as suspension cells
Once DCF-DA has diffused into cells it is deacetylated by cellular esterases to a non-fluorescent compound and rapidly oxidized by ROS into DCF. DCF is highly fluorescent and can be detected by microscopy, titration in microplate and also cytometry (ex 485/em 535). The fluorescence intensity that results is proportional to the ROS levels within the cell.