About this item
The IBI Fecal DNA Extraction kit is designed for rapid isolation of Genomic DNA from microorganisms, such as bacteria and fungi in stool samples.
- Sample Size: 180 – 220mg of fresh or frozen stool samples
- Utilizes a proprietary inhibitor removal buffer
- Includes Beadbeating tubes (containing ceramic beads) for sample homogenization, inhibitor removal columns, and Genomic DNA columns
- Isolated DNA is ready for PCR, restriction enzyme digestion and sequencing reactions
The IBI Fecal DNA Extraction kit is designed for rapid isolation of Genomic DNA from microorganisms, such as bacteria and fungi in stool samples. The stool sample is homeginized and disrupted using a lysis buffer and ceramic beads. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated with a proprietary Inhibitor removal buffer. Residual inhibitors are further removed by passing through a specialized PCR inhibitor removal column.
The extract procedure can be completed in 50 minutes and the purified genomic DNA is ready for use in PCR, restriction enzyme digestion and sequencing reactions.
Specifications
- Method / Format:Column (silica-based)Bead beating
- Prep size:Mini
- Refine target molecule:Total DNA
- Sample type:Stool
- Target molecule:DNA
- Description:Fecal DNA Kit