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Specifications
- Description:Colorimetric Nitric Oxide Assay Kit
- Size:1 kit
- Cat. No.:102994-820
- Supplier no.:NSC31-K01
Specifications
About this item
Nitric Oxide Synthase Colorimetricdetermines Nitric Oxide Synthase in biological fluids.
- Dynamic Range: 0.5 - 100 µM
- Sensitivity: 1 pmol/µL or ~1 µM
- Incubation: 5 minutes
The Nitric Oxide Synthase Colorimetric Assay kit employs immunoaffinity purified Nitrate Reductase (NaR) enabling the measure of total nitric oxide (NO) produced in in vitro experimental systems. Nitric oxide can be spectrophotometrically assayed by measuring the accumulation of its stable degradation products, nitrate and nitrite. The ratio of these two products in biological fluids, tissue culture media, etc. may vary substantially. Hence, for accurate assessment of the total nitric oxide generated, one must monitor both nitrate and nitrite. An excellent solution to this problem is the enzymatic conversion of nitrate to nitrite by the enzyme nitrate reductase, followed by quantitation of nitrite using Griess Reagent. In addition to providing all necessary components in a microtiter format, this kit employs affinity purified nitrate reductase and NADH, thereby circumventing some of the potential problems reported for NO measurement using NADPH dependent nitrate reductases.