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Specifications
- Description:Nitric Oxide Synthase Non-Enzymatic Assay Kit
- Size:1 kit
- Cat. No.:102994-816
- Supplier no.:NOS39-K01
Specifications
About this item
Nitric Oxide Synthase Colorimetric Non-Enzymatic detects Nitric Oxide Synthase in biological fluids.
- Dynamic Range: 0.5 - 100 µM
- Sensitivity: 1 pmol/µL or ~1 µM
- Incubation: 5 minutes
Nitric oxide can be spectrophotometrically assayed by measuring the accumulation of its stable degradation products, nitrate and nitrite. The ratio of these two products in biological fluids, tissue culture media, etc. may vary substantially. Hence, for accurate assessment of the total nitric oxide generated, one must monitor both nitrate and nitrite. An excellent solution to this problem involves the conversion of nitrate to nitrite catalyzed by cadmium. This is then followed by the quantitation of nitrite using the griess reaction. This kit is suitable for the quantitative determination of total nitric oxide in samples that have high protein concentrations. The cadmium catalyst proves far more robust when in the presence of harsh deproteinating reagents than the alternative, nitrate reductase.