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- Description:Ultra Sensitive Colorimetric Assay for Nitric Oxide Synthase
- Size:1 kit
- Cat. No.:102994-824
- Supplier no.:NSU03-K01
Nitric Oxide Synthase Ultra Colorimetric determines Nitric Oxide Synthase in biological fluids.
- Dynamic Range: 0.5 - 100 µM
- Sensitivity: 1 pmol/µL or ~1 µM
- Incubation: 1.5 hours
The traditional method for measuring nitric oxide synthase (NOS) activity is performed by radiochemical assay that measures the conversion of L-[3H]arginine to L-[3H]citrulline. This method is expensive and requires regulation of radioactive materials. The Ultrasensitive Colorimetric NOS Assay Kit is a low-cost novel assay that allows to detect NOS activity without the need for radioactivity. Our Ultrasensitive NOS Assay Kit employs a NADPH recycling system to allow NOS to operate linearly for hours as nitric oxide-derived nitrate and nitrite accumulate. NOS can be assayed spectrophotometrically by measuring the accumulation of its stable degradation products, nitrate and nitrite. The ratio of these two products in biological fluids, tissue culture media, etc. may vary substantially. Hence, for accurate assessment of the total nitric oxide generated, one must monitor both nitrate and nitrite. An excellent solution to this problem is the enzymatic conversion of nitrate to nitrite by the enzyme nitrate reductase (NaR), followed by quantization of nitrite using Griess Reagent. This kit allows for efficient high-throughout screening of NOS activity in resting cells or cell lysates as well as biological fluids and tissue homogenates. The kit is also ideal for in vitro NOS assays using recombinant purified NOS. All materials necessary to perform the entire assay in a 96-well microplate format are provided with the kit.