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Anti-KRT4, KRT5, KRT6A, KRT6B, KRT8, KRT10, KRT13, KRT18 Mouse Monoclonal Antibody [clone: SPM583]
  76195-388
 :  Prosci
Anti-KRT4, KRT5, KRT6A, KRT6B, KRT8, KRT10, KRT13, KRT18 Mouse Monoclonal Antibody [clone: SPM583]
  76195-388
 :  Prosci
 :  34-196

 

  • Antibody Type:
    Primary
  • Antigen Name:
    keratin 4, keratin 5, keratin 6A, keratin 6B, keratin 8, keratin 10, keratin 13, keratin 18
  • Antigen Symbol:
    KRT4, KRT5, KRT6A, KRT6B, KRT8, KRT10, KRT13, KRT18
  • Clonality:
    Monoclonal
  • Clone:
    SPM583
  • Conjugation:
    Unconjugated
  • Flow Cytometry:
    Yes
  • Host:
    Mouse
  • ImmunoChemistry:
    Yes
  • ImmunoFluorescence:
    Yes
  • Isotype:
    IgG1
  • Reactivity:
    Marmoset,
    Frog,
    Pig,
    Goat,
    Human,
    Bovine,
    Guinea pig,
    Rat,
    Mouse
  • Western Blot:
    Yes
  • Size:
    100 ug
  • Format:
    Purified Antibody
  • Form:
    Liquid
  • Gene ID:
    P19013, P13647, P02538, P04259, P48668, P05787, P13645, P13646, P05783
  • Antigen Synonyms:
    KRT4, KRT5, KRT6A, KRT6B, KRT8, KRT10, KRT13, KRT18
  • Storage Buffer:
    PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • Storage Temperature:
    Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
  • Concentration:
    0.2 mg/mL
  • Shipping Temperature:
    4 °C
  • Immunogen:
    A keratin-enriched preparation from cultured human A431 cells was used as the immunogen for this anti-Multi-Cytokeratin antibody.
  • Purification:
    Protein G affinity chromatography
  • Cat. No.:
    76195-388

 

 

This mAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml Western blot: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the anti-Multi-Cytokeratin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Type: Primary
Antigen: KRT4, KRT5, KRT6A, KRT6B, KRT8, KRT10, KRT13, KRT18
Clonality: Monoclonal
Clone: SPM583
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human, Bovine, Rat, Mouse, Guinea Pig, Frog, Goat, Marmoset, Pig