There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile. Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation. The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology. [Wiki]

Immunofluorescence: 1-2 ug/ml Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT (3) Prediluted IHC only format: incubate for 30 min at RT (2) Flow Cytometry: 0.5-1 ug/million cells in 0.1ml WB: 0.5-1 ug/ml Titering of the recombinant Acidic Cytokeratin antibody may be required for optimal performance.

1. This antibody will detect cytokeratins -10, -14, -15, -16, -19.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
3. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Type: Primary
Antigen: KRT77
Clonality: Monoclonal
Clone: KRTL/1577R
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG, k
Reactivity: Human