Specifications
- Antibody Type:Primary
- Antigen Name:Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
- Antigen Symbol:FCGR3A
- Clonality:Monoclonal
- Clone:HuNK2
- Conjugation:Unconjugated
- Flow Cytometry:Yes
- Host:Mouse
- ImmunoFluorescence:Yes
- Isotype:IgG2a kappa
- Reactivity:Human
- Western Blot:Yes
- Size:100 ug
- Neutralisation:Yes
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P08637
- Antigen Synonyms:FCGR3A
- Storage Buffer:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- Storage Temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0.2 mg/mL
- Shipping Temperature:4 °C
- Immunogen:Human peripheral blood lymphocytes from a NK-leukemia patient were used as the immunogen for the CD16 antibody.
- Tested Applications:Functional
- Purification:Protein G affinity chromatography
- Cat. No.:76193-968
Specifications
About this item
Recognizes a protein of 50-65kDa, identified as CD16 (Workshop IV; Code N39 ) (also known low affinity Fc receptor III for IgG (FcRIII) or Leu 11). CD16 exists as a polypepetide-anchored from (FCRIIIA or CD16A) on human natural killer (NK) cells and monocytes/ macrophages and as a glycosylphosphatidylinositol (GPI)-anchored form (FcRIIIB or CD16B) on neutrophils. CD16B is polymorphic and the two alleles are termed NA1 and NA2.3 CD16 plays a role in signal transduction, NK cell activation and antibody-dependent cellular cytotoxicity. This mAb has been showed to inhibit the binding of immune complex to NK cells, inhibit cytotoxicity of NK cells, and induce calcium fluxes in NK cells and neutrophils.
Functional Activity (Order SAF formulation) Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml Optimal dilution of the CD16 antibody should be determined by the researcher.
Type: Primary
Antigen: FCGR3A
Clonality: Monoclonal
Clone: HuNK2
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a, kappa
Reactivity: Human