Fast, sensitive DNA LAMP assays in a fully optimizable kit format.

• Loop-mediated isothermal amplification (LAMP): allows researchers to run assays outside of high-tech laboratories using low complexity instrumentation
• Reaction temperature (68 to 74 °C): reduces background amplification and improves primer specificity depending on the target
• Lyophilization-ready: component formulations enable generation of room temperature stable test kits through lyophilization
• Equivalent performance to the LavaLAMP™ DNA master mix
• When LavaLAMP™ DNA component kit and master mix reactions are formulated identically
• Provides a reference point, same results, when switching from the LavaLAMP™ DNA master mix to this kit for additional optimization experiments

The LavaLAMP™ DNA component kit is designed for loop-mediated isothermal amplification (LAMP) assay development. This kit contains a unique, highly processive DNA polymerase enzyme with strong strand displacement activity, making it well suited to LAMP and other isothermal amplification assays.

The enzyme in this kit is identical to the enzyme present in the LavaLAMP™ DNA master mixes (Cat. No. 75996-746, 75996-748), but the individual component format of these kits, as opposed to the master mix format, gives you complete control of each LAMP assay and full optimization capabilities. Importantly, these kits are an exact deconstructions of the LavaLAMP® DNA master mixes, and when reactions are setup using the initial experimental reaction conditions outlined in the component kit user manual, the composition of that reaction formulation matches the composition of the master mix-based reaction.

To aid in assay development and future lyophilization of LavaLAMP™-based reactions, all components in this kit are compatible with lyophilization. This feature means that once the decision is made to begin lyophilization of the reactions, no changes to the reaction components will be necessary.