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Specifications
- Conjugation:DNP(2,4-Dinitrophenol)
- Size:1 mg
- Storage Conditions:–20 °C
- Protein Synonyms:SrtA Peptide|Staphylococcus aureus transpeptidase Sortase A|Sortase
- Protein/Peptide Name:Bacterial Sortase Substrate III
- Purity:95%
- Molecular Weight:928 Da
- Sequence:Abz-LPETG-K(Dnp)-NH2
- Cat. No.:103007-528
- Supplier no.:AS-63717
Specifications
About this item
The Staphylococcus aureus transpeptidase Sortase A (SrtA) anchors virulence and colonization-associated surface proteins to the cell wall. SrtA selectively recognizes a C-terminal LPXTG motif. SrtA readily reacts with its native substrate Abz-LPETG-Dap(DNP)-NH2, cleaving it and catalyzing the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Cleavage of this FRET substrate can be monitored at Ex/Em=320 nm/420 nm.
Sequence:Abz-LPETG-K(Dnp)-NH2
MW:928 Da
% peak area by HPLC:95
Storage condition:-20° C