Pierce 1-Step™ NBT/BCIP Substrate Solution, Thermo Scientific
PI34042
:
- Description:1-Step™ NBT/BCIP Substrate Solution
- Size:250 mL
- Cat. No.:PI34042
- Application:Immunoblotting
- Supplier no.:34042
- No. of uses:25 uses at 10 ml each
The 1-Step™ NBT and BCIP substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
- Sensitive with low background and minimal assay-to-assay variability
- Ready-to-use single component
The combination of NBT and BCIP yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, an enzyme conjugate for antibody probes. Used for immunoblotting, immunohistochemistry, and in situ hybridization applications.