Pronase Reagent, Ready To Use, Electron Microscopy Science

About this item

Pronase is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

Antigen retriever for some antibodies in IHC
Please refer to primary antibody protocol for IHC

Pronase is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC.

Pronase digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.

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