Trypsin Kit, Electron Microscopy Science
About this item
Trypsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
- Use as antigen retriever for some antibodies in IHC
- Please refer to primary antibody protocol for IHC
- Supplied as concentrated form along with buffer
In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.
Delivery information: Small kit contains (15 mL) Reagent B Buffer and (2 mL) Reagent E (Enzyme).
Large kit contains (100 mL) Reagent B Buffer and (10 mL) Reagent E (Enzyme).
2 Options Available Below
- Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.
- Return Policy
Product Details & Documents
Trypsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
- Use as antigen retriever for some antibodies in IHC
- Please refer to primary antibody protocol for IHC
- Supplied as concentrated form along with buffer
In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.
Delivery information: Small kit contains (15 mL) Reagent B Buffer and (2 mL) Reagent E (Enzyme).
Large kit contains (100 mL) Reagent B Buffer and (10 mL) Reagent E (Enzyme).
Specifications for Product Family
Specifications
Specifications
- Catalog No:
- 103303-052
- 103305-614
- Storage Temperature:
- 2-8°C. DO NOT FREEZE.
Specifications
Product Family Options
Product Information
- AvailabilityPrice
- 103303-052Trypsin Kit SmallSupplier Number: 64142-01Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.103303-052Storage Temperature - 103305-614Trypsin Kit LargeSupplier Number: 64142-02Item requires temperature control for storage and delivery with additional fees. It's not eligible for return due to safety and quality concerns. Consider requirements before purchasing.Specifications:
More
Specifications
Cat. No.103305-614Storage Temperature2-8°C. DO NOT FREEZE.
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