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- Description:SensoLyte® pNPP Protein Phosphatase Assay Kit *Colorimetric*
- Size:1 Kit
- Environmentally Preferable:
- Storage Temperature:- 20 °C
- Cat. No.:103010-118
- No. of wells:96
- No. of assays/kit:500 assays (96 well plate) or 1250 assays (384 well plate)
- Supplier no.:AS-71105
p-Nitrophenyl phosphate (pNPP) is proven to be an effective chromogenic substrate for protein tyrosine phosphatases and serine/threonine phosphatases
Protein phosphatases have received great attention as drug-screening targets. SensoLyte® Protein Phosphatase Assay Kits uses specific substrates to quantify specific protein phosphatase activities such as MFP, pNPP FDP or acid phosphatases. The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of protein phosphatase inhibitors/inducers.
Optimized to detect protein phosphatase activity; compatible with HTS; provides enough reagents to perform 500 assays in a 96-well format; minimal hands-on time; detailed protocol and references provided.
SensoLyte® pNPP Protein Phosphatase Assay Kit uses pNNP to quantify protein phosphatase activities. The kit can be used for characterizing kinetics of enzyme reaction and high throughput screening of protein phosphatase inhibitors. It has high sensitivity and wide linear range (The detection limit is generally 3 ng or below).