Easy, reliable isolation of high-quality fosmid DNA

The FosmidMAX™ DNA Purification Kit was developed for easy, reliable isolation of high-quality fosmid DNA

The FosmidMAX kit is ideal for the purification of induced fosmids produced by the CopyControl™ Fosmid Library Production Kit. The CopyControl cloning technology enables the user to grow the fosmid clones at single copy to ensure insert stability and cloning of toxic gene products and then induce the clones to high-copy number for high yields of DNA. Using the FosmidMAX Kit, up to 4 µg of fosmid DNA can be isolated from 1.5 ml of an induced clone.

Isolate up to 0.6, 15, or 25 µg of fosmid DNA from 1.5-, 40-, or 100-ml cultures of a single-copy fosmid, respectively, with an easy to use protocol
Maximize yields because the FosmidMAX kit does not use columns or binding resins that reduce yields
Reduce costs by using only the reagents you need based on the amount of DNA being purified
Purify DNA from single-copy fosmid clones, induced CopyControl fosmid and cosmid clones
Reduce waste and use of hazardous reagents because no columns or toxic organic solvents are used

The scalable protocol is based on a modified alkaline-lysis procedure that typically yields 0.6-25 µg of fosmid DNA from 1.5 to 100 ml of a single-copy fosmid culture. Selective precipitation steps and the incorporation of the Epicentre RiboShredder™ RNase Blend effectively remove contaminants that degrade DNA and interfere with downstream applications.