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- Size:100 mL
- Form:Liquid
- Storage Temperature:4°C
- Brand Name:Genemed
- Shipping Temperature:4°C
- Cat. No.:75814-850
- Supplier no.:10-0037
For In Vitro Diagnostic Use.
This product is intended to be used for the heat induced epitope (or antigen) retrieval (HIER) of formalin-fixed, paraffin-embedded (FFPE) tissues prior to immunohistochemical (IHC) staining.
One bottle of Tris EDTA Buffer, pH 9 containing 200 mM Tris and 20 mM EDTA.
Formalin fixation forms protein cross-links that mask the antigenic sites in tissue specimens, thereby giving weak or false negative staining for IHC detection of certain proteins. Tris EDTA Buffer, pH 9 is designed to break the protein cross-links, thus unmasking the antigens and epitopes in FFPE tissue sections and enhancing staining intensity of many antibodies.
For use after de-parafinnizing and rehydrating slides. If necessary, block endogenous peroxidase activity before HIER step.
1. Make 1X Tris EDTA Buffer using 1 part 20X Tris EDTA Buffer and 19 parts Reagent Water.
2. Wash slides in 3 changes of 1X PBS or reagent water to remove alcohol / peroxidase block.
3. Place slides in appropriate sized slide container and fill with 1X Tris EDTA Buffer. Make sure section is immersed.
4. Incubate for 20-40 minutes at temperature ≥ 95°C.
Note: Optimal incubation time in variable heating source should be determined by user.