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QuickExtract™ FFPE DNA Extraction Solution, PCR-ready DNA from FFPE Samples, Biosearch Technologies
QuickExtract™ FFPE DNA Extraction Solution, PCR-ready DNA from FFPE Samples, Biosearch Technologies
Catalog # 76081-770
Supplier:  Lucigen
CAS Number:  
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QuickExtract™ FFPE DNA Extraction Solution, PCR-ready DNA from FFPE Samples, Biosearch Technologies
Catalog # 76081-770
Supplier:  Lucigen
Supplier Number:  QEF81050
CAS Number:  
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Specifications

  • Application:
  • Method / Format:
    Extraction for direct PCR
  • Prep size:
    Mini
  • Refine target molecule:
    Total DNA
  • Sample type:
    FFPE tissues
  • Target molecule:
    DNA
  • Description:
    QuickExtract™ FFPE DNA Extraction Kit
  • Size:
    50 mL
  • Environmentally Preferable:
  • Description:
    QuickExtract™ FFPE DNA Extraction Kit
  • Cat. No.:
    76081-770

Specifications

About this item

Simple, rapid extraction (no purification) of PCR-ready DNA from challenging FFPE tissue samples

  • Fast: PCR-ready DNA in minutes, not days
  • Simple: No spin columns or transfer steps are required
  • Compatible: Extracted DNA is compatible with both real-time and endpoint PCR
  • Safe: No xylene or phenol extractions used

The analysis of nucleic acid from formalin-fixed, paraffin-embedded (FFPE) specimens is challenging due to the extensive cross-linking of all tissue components during the fixation process

The QuickExtract™ FFPE DNA Extraction Kit is a fast, simple, and inexpensive method for preparing genomic DNA for PCR amplification from archival samples. The protocol requires only heat treatment to melt the paraffin, lyse the cells, decrease the fomalin-induced cross-linking in the sample, and degrade compounds inhibitory to amplification. Following heat treatment, the DNA sample is ready for PCR.

These challenges include chemical modification of the DNA, cross-linking of DNA with other molecules, degradation of the DNA, and the limited amount of nucleic acid in the samples.

Certifications: For research use only. Not for human or diagnostic use.