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Specifications
- Method / Format:Extraction for direct PCR
- Prep size:Mini
- Refine target molecule:Total DNA
- Sample type:FFPE tissues
- Target molecule:DNA
- Description:QuickExtract™ FFPE DNA Extraction Kit
- Size:50 mL
- Description:QuickExtract™ FFPE DNA Extraction Kit
- Cat. No.:76081-770
Specifications
About this item
Simple, rapid extraction (no purification) of PCR-ready DNA from challenging FFPE tissue samples
- Fast: PCR-ready DNA in minutes, not days
- Simple: No spin columns or transfer steps are required
- Compatible: Extracted DNA is compatible with both real-time and endpoint PCR
- Safe: No xylene or phenol extractions used
The analysis of nucleic acid from formalin-fixed, paraffin-embedded (FFPE) specimens is challenging due to the extensive cross-linking of all tissue components during the fixation process
The QuickExtract™ FFPE DNA Extraction Kit is a fast, simple, and inexpensive method for preparing genomic DNA for PCR amplification from archival samples. The protocol requires only heat treatment to melt the paraffin, lyse the cells, decrease the fomalin-induced cross-linking in the sample, and degrade compounds inhibitory to amplification. Following heat treatment, the DNA sample is ready for PCR.
These challenges include chemical modification of the DNA, cross-linking of DNA with other molecules, degradation of the DNA, and the limited amount of nucleic acid in the samples.