To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
- Species:E. coli
- Size:20000 units
- Enzyme Name:Exonuclease I
- Concentration:20 units/μl
- Cat. No.:76081-722
Digest single-stranded DNA from the 3' to 5' end, but not double-stranded or dsDNA with short 3' overhangs
- Specific: Processively digest ssDNA in a 3´ to 5´ direction without harming dsDNA - short 3´ overhangs in DNA are not digested
- Flexible: Active under a wide variety of buffer conditions and can be added directly into most reaction mixes
- Heat Inactivated: Destroy the enzyme activity by incubating at 80°C for 15 minutes
Exonuclease I digests ssDNA in a 3´ to 5´ direction,1-3 but does not digest dsDNA
Applications include:
Removal of residual ssDNA, including oligos, from reaction mixes
Removal of ssDNA from nucleic acid mixtures
Although it requires the presence of magnesium and a free 3´-hydroxyl terminus for activity, it is active under a wide variety of buffer conditions and can be added directly into most reaction mixes. Exonuclease I can be heat-inactivated by incubation at 80°C for 15 minutes.