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Specifications
- Size:3000 units
- Enzyme Name:T4 Polynucleotide Kinase
- Concentration:10 units/μl
- Cat. No.:76081-708
Specifications
About this item
Phosphorylate 5' hydroxy ends of ssDNA and dsDNA, RNA, and nucleoside 3´ monophosphates
- Robust: Highly active enzyme that readily phosphorylates 5' hydroxl ends of nucleic acids
- Flexible: Use newly phosphorylated nucleic acids in a variety of applications such as cloning, next gen sequencing library prep, and preparation of labeled nucleic acids
T4 Polynucleotide Kinase (PNK) catalyzes the transfer of the γ-phosphate from ATP to the 5´ hydroxyl of ssDNA and dsDNA, RNA, and nucleoside 3´ monophosphates
Applications include:
Labeling of 5´ termini of DNA and RNA with 32P or 33P for DNA sequencing, blot-hybridization experiments, or transcript mapping using Mung Bean Nuclease, S1 nuclease, or other nucleases
Phosphorylation of oligonucleotide linkers and other DNA or RNA molecules prior to ligation, or for use in ligation amplification reactions with Ampligase® Thermostable DNA Ligase
Preparation of labeled DNA or RNA molecular weight markers for gel electrophoresis and chromatography
The enzyme also removes the 3´ phosphate from 3´-phosphoryl polynucleotides, deoxyribonucleoside 3´ monophosphates, and deoxyribonucleoside-3´,5´-diphosphates to form a 3´-hydroxyl group.