Single dropout of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media.

• Transcription Regulation
• Time tested formulation is available in complete and dropout formulations
• For use with DOB or YNB with the appropriate nitrogen and carbon sources to custom formulate your own selective Synthetic Defined yeast media

Single Dropout Complete Supplement Mixture used to select for yeast containing plasmids with specific nutritional markers and to select for gene function in transformation experiments.

Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the DOB or DOBA with CSM-Arg formula. DOB (Drop Out Base) is 1.7 g Yeast Nitrogen Base, 5 g Ammonium Sulfate, 20 g Dextrose per liter. DOBA is DOB with 17% agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare liquid plate medium by adding 43.7 g DOBA powder and 0.74g of CSM-Arg to a liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121 °C for 25 minutes. After cooling to 50 °C plates were poured.