The pET-23a-d(+) vectors carry an N-terminal T7 Tag® sequence plus an optional C-terminal His Tag® sequence.

• Vectors differ from pET-21a-d(+) by the "plain" T7 promoter instead of the T7 lac promoter and by the absence of the lac I gene
• Sequence is numbered by the pBR322 convention
• The T7 expression region is reversed on the vector map (TB051)

The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock).