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Specifications
- Host:
- Description:Nick translation DNA Labeling System 2.0
- Size:50 tests
- Environmentally Preferable:
- Cat. No.:76013-836
Specifications
About this item
Nick translation system for preparing FISH probes
- Ready-to-use Nick Translation Enzyme Mix minimizes pipetting error
- Short labeling time - only 1 hour
- Validated with fluorophore, biotin, and digoxigenin labeled dUTPs
The Nick Translation DNA Labeling System 2.0 provides a simple and efficient method for generating labeled DNA
The Nick Translation DNA Labeling System 2.0 can accommodate a wide range of fluorophore-labeled, biotin-labeled, and digoxigenin-labeled nucleotides. In addition to choice of label, the kit design allows the user to optimize incorporation and product size by adjusting the ratio of labeled-dUTP to dTTP. The ready-to-use NT Enzyme Mix is user friendly and minimizes error from pipetting.
Probes labeled by nick translation can be used in many different hybridization techniques including: in situ hybridization (ISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and reassociation kinetic studies.
Contains NT Enzyme Mix 250 µL, Reaction Buffer 250 µL, dNTP Mix (dATP, dGTP, dCTP) 250 µL, dTTP 180 µL, Stop Buffer 250 µL, Nuclease-free Water 5 mL.