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Specifications
- CAS No.:9002-07-7
- Source:Pancreas
- Species:Pig
- Size:20 g
- Environmentally Preferable:
- Form:White powder
- MDL No.:MFCD00082094
- Enzyme Name:Trypsin
- Solubility:Soluble in water; practically insoluble in alcohol and glycerol.
- Cat. No.:75875-604
- Supplier no.:0210313920
Specifications
About this item
Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues
- pH: ~ 8.0 (optimum) (Lit.)
Trypsin is used to re-suspend cells adherent to the cell culture dish wall during the process of harvesting cells. Trypsin can also be used to dissociate dissected cells. Trypsin can be used to break down casein in breast milk. Trypsin is commonly used in biological research during proteomics experiments to digest proteins into peptides for mass spectrometry analysis. Trypsin can also be used to dissolve blood clots in its microbial form and treat inflammation in its pancreatic form.
The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. The 1:250 designation indicates the tryptic activity based on standardized testing. This ratio indicates that one part trypsin will digest 250 parts casein in the USP activity assay. Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain.